Full-length Transcriptome Sequencing and Analysis Service of Yeast

The complete transcript includes the sequence from the 5'end to the 3'end of the polyA tail, and the length is concentrated in 1-6 kb. Due to the short read length of the second-generation sequencing technology, the sequence fragments obtained need to be spliced, and the resulting transcripts may have splicing errors and more chimeras, so that a complete transcript cannot be obtained. However, the third-generation sequencing is based on the Pacbio platform, using single-molecule real-time sequencing technology (SMRT), due to its ultra-long read length (average 15kb), you can directly obtain a complete full-length transcript without splicing. Therefore, the problem of short splicing of transcripts and incomplete information of species without reference genome is overcome, and the study of structural variations such as variable splicing and fusion genes in species with reference genome can be realized.

For yeast without a reference sequence, full-length transcriptome sequencing can construct a high-quality gene set, laying the foundation for subsequent functional studies of yeast. For yeasts with reference sequences, new transcripts can be discovered, reference gene sets can be improved, and structural variations such as alternative splicing and gene fusion can be identified.

Service introduction

Based on the yeast high-throughput sequencing technology platform, Creative BioMart launched full-length transcriptome sequencing and analysis service for yeast. If you have relevant requirements, please contact us.

Experiment procedure

Sample requirements

• Total RNA ≥ 10 μg, concentration ≥ 300 ng / μl, RIN ≥ 8.0, OD260 / 280 = 1.6~2.2，OD260 / 230= 1.4~2.5
• Yeast cell ≥ 2×10⁷ ~ 2×10⁸

Please put the sample in a 1.5 ml tube, indicate the name, concentration and preparation time of the sample on the tube, and seal the tube with parafilm. Before shipping, fix all sample tubes in 50 ml centrifuge tubes with caps and ship them on dry ice.

Analysis content

• Standard information analysis

• Customized information analysis

1) Correction of the second-generation data to the third-generation data
2) Gene quantification and differential expression analysis
3) Multi-omics association analysis

Project cycle

• The operating cycle of the standard process is 90 days.

Delivery content

• Raw data
• Project analysis report, including experimental equipment, reagents, methods, results, conclusions.

Service process

Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.

Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.