Dual RNA-seq Service of Yeast

General interaction methods include symbiosis, parasitism, competition and predation. Dual RNA-seq targets two or more interacting species. Ordinary transcriptomes need to separate two species, but will waste part of the information and bring other impacts, while Dual RNA-seq can study two or more species at the same time, without separation, eliminating the impact of separation. The cost is lower, and only one transcriptome library can be constructed, and two species can be analyzed at the same time, and finally the regulatory relationship between the genes of the two species can be obtained.

Yeast is the earliest microorganism to be used. It generally refers to various single-cell fungi that can ferment sugars used in brewing and production, and can also be pathogenic bacteria used in genetic engineering and cell cycle research. Yeast also participates in the interaction of symbiosis, parasitism, and competition with other organisms. For example, yeast and lactic acid bacteria have a synergistic effect in fermented products. In order to specifically study these interaction methods, Dual RNA-seq is strongly recommended. It can study two or more species at the same time, without separation, to help you achieve your goals.

Service introduction

Based on the yeast high-throughput sequencing technology platform, Creative BioMart launched the yeast Dual RNA-seq analysis service. If you have relevant requirements, please contact us.

Sample requirements

• Total RNA ≥ 60 μg, concentration ≥ 100 ng / μl, RIN ≥ 7.5

Please put the sample in a 1.5 ml tube, indicate the name, concentration and preparation time of the sample on the tube, and seal the tube with parafilm. Before shipping, fix all sample tubes in 50 ml centrifuge tubes with caps and ship them on dry ice.

Library construction strategy

• Yeast (eukaryotic) - eukaryotic interaction

Use ployA enrichment method to construct library.

• Yeast (eukaryotic) - prokaryotic interaction

The library is constructed by removing rRNA strand specificity.

Experiment procedure

Analysis content

• Standard analysis

Standard eukaryotic / prokaryotic transcriptome analysis

• Advanced analysis

WGCNA analysis
String database annotation
PHI database annotation
VFDB database annotation
HPIDB database annotation

Project cycle

• The operating cycle of the standard process is 50 working days.

Delivery content

• Raw data
• Project analysis report, including experimental equipment, reagents, methods, results, conclusions.

Service process

Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.

Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.