CircRNA Sequencing and Analysis Service of Yeast

Yeast, as a widely used model organism, is of great significance to food, medicine and industrial  production. At present, the research on yeast coding genes is more in-depth and extensive, but the research on circRNA is still in its infancy.

Circular RNA (circRNA) is a special type of non-coding RNA molecule, and it is also the latest research hotspot in the RNA field. Unlike traditional linear RNA (linear RNA, containing 5'and 3'ends), circRNA molecules are in a closed loop structure, which is not affected by RNA exonuclease, and has more stable expression and is not easily degraded. Research in recent years has shown that circRNA has an important regulatory role in the growth and development of organisms and the resistance to the external environment. CircRNA has many functions and can be used as a sponge of miRNA to competitively bind to miRNA and play the function of ceRNA to regulate gene expression. In recent years, it has also been found to be closely related to the occurrence and development of diseases, and has great application prospects in the direction of disease diagnosis markers.

Service introduction

Creative BioMart has launched high-throughput sequencing and analysis services for circRNA in yeast, which can identify circular RNA in yeast and analyze the 'sponge effect' of circRNA-miRNA, and realize research on the mechanism of ceRNA (competing endogenous RNAs). It can be applied to the construction of circRNA expression profile in yeast, the study of the mechanism of circRNA in yeast and the development of disease biomarkers. If you have related needs, please contact us.

Application fields of yeast circRNA

• Research on the pathogenesis of tumors and other diseases
• Gene expression and regulation during growth and development
• Other research directions concerned with gene transcription, expression and regulation

Sample requirements

• Total RNA ≥ 15 μg, concentration ≥ 100 ng / μl, RIN ≥ 7.5

Please put the sample in a 1.5 ml tube, indicate the name, concentration and preparation time of the sample on the tube, and seal the tube with parafilm. Before shipping, fix all sample tubes in 50 ml centrifuge tubes with caps and ship them on dry ice.

Experiment flow

Analysis content

• Standard information analysis

1) Basic statistics
2) circRNA prediction and identification
3) Annotation of the linear gene where the circRNA is located
4) circRNA location analysis on the genome
5) Quantitative analysis of circRNA expression
6) Analysis of expression differences between samples (groups)

• Advanced information analysis

1) Functional annotation and enrichment analysis of GO / Pathway of genes derived from differential circRNA

Project cycle

• The operating cycle of the standard process is 55 working days.

Delivery content

• Raw data
• Project analysis report, including experimental equipment, reagents, methods, results, conclusions.

Service process

Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.

Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.