Acetylation Proteomic Analysis of Yeast

Acetylation is a highly conserved and reversible protein modification in vivo, which plays a very important role in the activation of transcriptional regulators in the nucleus. In addition, there are a large number of non-histone acetylation modifications involved in the regulation of metabolic pathways and metabolic enzyme activities. Due to the low content and wide dynamic range of acetylated proteins in biological samples, acetylated peptides need to be enriched before mass spectrometry analysis to improve their abundance, and then traditional quantitative proteome analysis methods are used to analyze the enriched acetylated peptides Quantitative analysis of peptide samples.

Creative BioMart optimized the experimental method, using peptide pre-separation to reduce the impact of high-abundance histones on protein acetylation identification, and then combined with co-immunoprecipitation to enrich acetylated peptides through efficient antibodies, so as to achieve large-scale acetylation identification and quantitative. 

Service introduction

Creative BioMart uses mass spectrometry platform (Q Exactive HF, Orbitrap Fusion, Orbitrap Fusion Lumos) combined with nano LC - MS / MS to launch a service package for yeast acetylation quantitative proteome analysis. You only need to tell us your experimental purpose and send us your samples, and we will take care of all the follow-up matters, including yeast protein extraction, protease digestion, acetylated peptide enrichment, peptide separation, MS analysis, MS raw data analysis and bioinformatics analysis. If you have any needs in this regard, please feel free to contact us.

Provided by clients

• Yeast ≥ 200 mg (dry weight)
• Yeast cell number ≥ 5×10⁷ 
• Yeast protein extract ≥ 1 mg, concentration > 2 mg / ml. Samples should not contain components that would interfere with separation, such as nucleic acids, lipids, and polysaccharides.
• Sample information sheet (the source, content, status and other basic information of the sample).

Experiment procedure

Delivery content

Creative BioMart will provide you with detailed technical reports including:

• Experimental procedure
• Relevant mass spectrometry parameters
• Details of the identified acetylation sites
• Mass spectrometry
• Raw data

Project cycle

• The operating cycle of the standard process is 45 working days.

Service process

• You can tell us your needs through online inquiry or contact us directly
• Our experts quickly produce alternative solutions
• Sign contract and send samples
• One-stop service
• Project final report

Confidentiality statement

Creative BioMart has many business partners and understands the importance of confidentiality to these companies. We take confidentiality very seriously, sign a non-disclosure agreement before communicating with you, and employ multiple firewalls and safeguards to protect your privacy.

Creative BioMart's experienced technicians are well-positioned to optimize protocols to your requirements and ensure high-quality protein acetylation analysis results. If you have specific related service needs, please contact us.